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PGL3 Vector Map

This post categorized under Vector and posted on October 25th, 2018.

Plasmid pGL3 BRE Luciferase from Dr. Peter ten Dijkes lab contains the insert Id1-BRE and is published in J Biol Chem. 2002 Feb 15277(7)4883-91. Epub 2001 Nov 29. This plasmid is available through Addgene.Plasmid pUC57-sgRNA expression vector from Dr. Xingxu Huangs lab contains the insert sgRNA scaffold and is published in Nat Methods. 2014 Mar 2. doi 10.1038nmeth.2857. This plasmid is available through Addgene.Plasmid pCP20 problem - (reply 1) Creating empty vector - (reply 5) cloning vector and restriction mapping - (reply 2) software to replace Vector NTI and a simple cloning problem - (reply 3) Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply 3) plasmid - (reply 2) no expression in pET 21 vector - (reply 2) DNA purification - Touble with isolating

Potencial promoter seqences vectorysis -strange 5 sequence - Qustions that arised in my investigation of promoter seqence (reply 3) CMV promoter - (reply 1) pls recommend a serum independent expression promoter for mammalian cells - (reply 1) promoter sequence - (reply 1) promoter cloning - (reply 1) how to find promoter - (reply 3) aligning promoter regions to find a binding site

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